Great company in Flavor and Fragrance industry - Research Investigator IFF Employee Review

Creation of taste receptor cell lines and evaluation was the biggest challenge since all sweet and umami receptors with at least two subunits and a G-protein, so it was hard to create a cell line with good response in taste assay, especially in HTS evaluation. I tried different vectors, different vector combinations, and three gene in separate vectors or three genes in one vector, finally I got some working stable cell lines. From this, I got a lot of experience in how to create a good stable cell lines and how to evaluate them.